A smooth muscle-specific monoclonal antibody recognizes smooth muscle actin isozymes.
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چکیده
منابع مشابه
A smooth muscle-specific monoclonal antibody recognizes smooth muscle actin isozymes
Injection of chicken gizzard actin into BALB/c mice resulted in the isolation of a smooth muscle-specific monoclonal antibody designated CGA7. When assayed on methanol-Carnoy's fixed, paraffin-embedded tissue, it bound to smooth muscle cells and myoepithelial cells, but failed to decorate striated muscle, endothelium, connective tissue, epithelium, or nerve. CGA7 recognized microfilament bundle...
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A monoclonal antibody (anti-alpha sm-1) recognizing exclusively alpha-smooth muscle actin was selected and characterized after immunization of BALB/c mice with the NH2-terminal synthetic decapeptide of alpha-smooth muscle actin coupled to keyhole limpet hemocyanin. Anti-alpha sm-1 helped in distinguishing smooth muscle cells from fibroblasts in mixed cultures such as rat dermal fibroblasts and ...
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متن کاملSimilarities and Differences in Smooth Muscle a-Actin Induction by TGF-b in Smooth Muscle Versus Non–Smooth Muscle Cells
Transforming growth factor-b (TGF-b) has been shown to stimulate smooth muscle (SM) a-actin expression in smooth muscle cells (SMCs) and non-SMCs. We previously demonstrated that the 2 CArG boxes A and B and a novel TGF-b control element (TCE) located within the first 125 bp of the SM a-actin promoter were required for TGF-b inducibility of SM a-actin in SMCs. The aims of the present study were...
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ژورنال
عنوان ژورنال: The Journal of Cell Biology
سال: 1985
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.100.3.807